Curriculum vitae

  Miguel Prudêncio

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Miguel Prudencio's citations
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Publications

Theses

M. Prudêncio (2000) "Molecular Enzymology of the Copper-Containing Enzymes Involved in Denitrification", PhD Thesis, University of East Anglia

Book Chapters

2. A.M. Mendes, A. Scholzen, A.K. Mueller, S.M. Khan, R.W. Sauerwein, M. Prudêncio (2017) “Whole Organism Pre-Erythrocytic Vaccines”, In: Rodriguez, A. and Mota M.M. (Eds) Malaria: immune response to infection and vaccination, Springer International Publishing, Cham, Switzerland

1. M. Prudêncio, C.D. Rodrigues, M.M. Mota (2006) “The relevance of host genes in Malaria”, In: Parrington,J. and Coward, K. (Eds) Comparative Genomics and Proteomics in the Identification of New Drug Targets, Taylor & Francis, Oxford, UK, SEB Exp. Biol. Ser., 58, 47-91

Scientific Articles

77. R. Azevedo, M. Markovic, M. Machado, B. Franke-Fayard, A.M. Mendes, M. Prudêncio (2017) "A bioluminescence method for in vitro screening of Plasmodium transmission-blocking compounds", Antimicrob. Agents Chemother., in press

76. K. Singh, J. Okombo, C. Brunschwig, F. Ndubi, L. Barnard, C. Wilkinson, P.M. Njogu, M. Njoroge, L. Laing, M. Machado, M. Prudêncio, J. Reader, M. Botha, S. Nondaba, L.M. Birkholtz, S. Lauterbach, A. Churchyard, T.L. Coetzer, J.N. Burrows, C. Yeates, P. Denti, L. Wiesner, T.J. Egan, S. Wittlin, K. Chibale (2017) "Antimalarial pyrido[1,2-a]benzimidazoles: Lead optimization, parasite life cycle stage profile, mechanistic evaluation, killing kinetics and in vivo oral efficacy in a mouse model", J. Med. Chem., 60, 1432-1448


75. C.S. Cunha§, B. Nyboer§, K. Heiss, M. Sanches-Vaz, D. Fontinha, E. Wiedtke, D. Grimm, J.M. Przyborski, M.M. Mota, M. Prudêncio*, A.K. Mueller* (2017) "P. berghei EXP-1 Interacts with Host Apolipoprotein H during Plasmodium Liver Stage Development", Proc. Natl. Acad. Sci. USA, 114, E1138-E1147. §- Equally contributing authors; *- Corresponding authors

74. A.M. Mendes, I.S. Albuquerque, M. Machado, J. Pissarra, P. Meireles, M. Prudêncio (2017) "Inhibition of Plasmodium liver infection by ivermectin", Antimicrob. Agents Chemother., 61, e02005-16


73. M.A.S. Cavadas, M.P. Monopoli, C.S. Cunha, M. Prudêncio, E. Pereira, I. Lynch, K.A. Dawson, R. Franco (2016) "Unravelling Malaria Antigen Binding to Antibody-Gold Nanoparticle Conjugates", Particle & Particle Systems Characterization, 33, 906-915


72. Carrasco M.P.*, Machado M., Gonçalves L., Sharma M., Gut J., Lukens A.K., Wirth D.F., André V., Duarte M.T., Guedes R.C., Dos Santos D., Rosenthal P.J., Mazitschek R., Prudêncio M.*, Moreira R. (2016) "Probing the Azaaurone Scaffold against the Hepatic and Erythrocytic Stages of Malaria Parasites", ChemMedChem, 11, 2194-2204  (* Corresponding authors)


71. P. Meireles, J. Sales-Dias, C.M. Andrade, J. Mello-Vieira, L. Mancio-Silva, J.P. Simas, H.M. Staines, M. Prudêncio (2016) "GLUT1-mediated glucose uptake plays a crucial role during Plasmodium hepatic infection", Cell. Microbiol., doi:10.1111/cmi.12646


70. R. Ferraz, J. Noronha, F. Murtinheira, F. Nogueira, M. Machado, M. Prudêncio, S. Parapini, S. D’Alessandro, C. Teixeira, A. Gomes, C. Prudêncio, P. Gomes (2016) "Primaquine-based ionic liquids as a novel class of antimalarial hits", RSC Advances, 6, 56134-56138


69. T.S. Macedo, L.C. Vegas, M. Paixão, M. Navarro, B.C. Barreto, P.C.M..Oliveira, S.G. Macambira, M. Machado, M. Prudêncio, S. D’Alessandro, N. Basilico, D.R.M. Moreira, A.A. Batista, M.B.P. Soares (2016) "Chloroquine-containing organoruthenium complexes are fast-acting multistage antimalarial agents", Parasitology, 143, 1543-1556


68.  C.J.A. Ribeiro, M. Espadinha, M. Machado, J. Gut, L.M. Gonçalves, P.J. Rosenthal, M. Prudêncio, R. Moreira, M.M.M. Santos (2016) "Novel squaramides with in vitro liver stage antiplasmodial activity", Bioorg. Med. Chem., 24, 1786-1792


67. N. Terzić, J. Konstantinović, M. Tot, J. Burojević, O. Djurković-Djaković, J. Srbljanović, T. Štajner, T. Verbić, M. Zlatović, M. Machado, I.S. Albuquerque, M. Prudêncio*, R.J. Sciotti, S. Pecic, S. D’Alessandro, D. Taramelli, B.A. Šolaja* (2016) "Re-investigating old pharmacophores: Are 4-aminoquinolines and tetraoxanes potential two-stage antimalarials?", J. Med. Chem., 59, 264-281 (* Corresponding authors)


66. I. Gomes, A. Reis, E. Pereira, E. Fortunato, M. Prudêncio, R. Franco (2015) "Nanoimunoensaios para diagnóstico de malária", An. Inst. Hig. Med. Trop., 14, 21-30


65. N.A.L. Pereira, Â. Monteiro, M. Machado, J. Gut, E. Molins, M.J. Perry, J. Dourado, R. Moreira, P.J. Rosenthal, M. Prudêncio,* and M.M.M. Santos* (2015), "Enantiopure Indolizinoindolones with in vitro Activity against Blood- and Liver-Stage Malaria Parasites". ChemMedChem, 10, 2080-2089 (* Corresponding authors)


64. A. Gomes, M. Machado, L. Lobo, F. Nogueira, M. Prudêncio, C. Teixeira, P. Gomes (2015) "N-cinnamoylation of antimalarial classics: Effects of using acyl groups other than cinnamoyl toward dual-stage antimalarials", ChemMedChem, 10, 1344-1349


63. H. Kaur, M. Machado, C. de Kock, P. Smith, K Chibale, M Prudêncio, K. Singh (2015) "Primaquineepyrimidine hybrids: Synthesis and dual-stage antiplasmodial activity", European Journal of Medicinal Chemistry, 101, 266-273


62. M.D. Lewis, J. Behrends, C.S. Cunha, A.M. Mendes, F. Lasitschka, K. Heiss, T.W.A. Kooij, M.Prudêncio, F.Frischknecht and A.-K. Mueller (2015), "Chemical attenuation of Plasmodium in the liver modulates severe malaria disease progression", Journal of Immunology, 194, 4860-4870


61. C.A.B. Rodrigues, R.F.M. Frade, I.S. Albuquerque, M.J. Perry, J. Gut, M. Machado, P.J. Rosenthal, M. Prudêncio, C.A.M. Afonso, R. Moreira (2015), "Targeting the Erythrocytic and Liver Stages of Malaria Parasites with s-Triazine-Based Hybrids", ChemMedChem, 10, 883-890


60. P. Liehl, P. Meireles, I.S. Albuquerque, M. Pinkevych, F. Baptista, M.M. Mota, M.P. Davenport, M. Prudêncio (2015), "Innate immunity induced by Plasmodium liver infection inhibits malaria reinfections", Infection and Immunity, 83, 1172-1180


59. C. Ramalhete, F.P. da Cruz, I.J. Sousa, S. Mulhovo, M.X. Fernandes, M. Prudêncio, M.-J.U. Ferreira (2014), "Dual-stage triterpenoids from an African medicinal plant targeting the malaria parasite", Bioorganic & Medicinal Chemistry, 22, 3887-3890


58. R. Oliveira, R.C. Guedes, P. Meireles, I.S. Albuquerque, L.M. Gonçalves, E. Pires, M.R. Bronze, J. Gut, P.J. Rosenthal, M. Prudêncio, R. Moreira, P.M. O’Neill, F. Lopes (2014), "Tetraoxane-Pyrimidine Nitrile Hybrids as Dual Stage Antimalarials", J. Med. Chem., 57, 4916-4923


57. D. Miranda, R. Capela, I.S. Albuquerque, P. Meireles, I. Paiva, F. Nogueira, R. Amewu, J. Gut, P.J. Rosenthal, R. Oliveira, M.M. Mota, R. Moreira, F. Marti, M. Prudêncio, P.M. O’Neill, F. Lopes (2014) "Novel Endoperoxide-Based Transmission-Blocking Antimalarials with Liver- and Blood-Schizontocidal Activities", ACS Med. Chem. Lett., 5, 108-112


56. A. Gomes, B. Pérez, I. Albuquerque, M. Machado, M. Prudêncio, F. Nogueira, C. Teixeira, P. Gomes (2014) "N-cinnamoylation of antimalarial classics: new quinacrine analogues with reduced toxicity and dual-stage activity", ChemMedChem, 9, 305-310


55. P. Liehl, V. Zuzarte-Luís, J. Chan, T. Zillinger, F. Baptista, D.l Carapau, M. Konert, K. Hanson, C. Carret, C. Lassnig, M. Müller, U. Kalinke, M. Saeed, A.F. Chora, D.T. Golenbock, B. Strobl, M. Prudêncio, L.P. Coelho, S.H. Kappe, G. Superti-Furga, A. Pichlmair, A.M. Vigário, C.M. Rice, K.A. Fitzgerald, W. Barchet, M.M. Mota (2014) "Host cell sensors for Plasmodium activate innate immunity against liver stage infection", Nature Medicine, 20, 47-53

54. A.S. Ressurreição, D. Gonçalves, A.R. Sitoe, I.S. Albuquerque, J. Gut, A. Góis, L.M. Gonçalves, M.R. Bronze, T. Hanscheid, G.A. Biagini, P.J. Rosenthal, M. Prudêncio , P. O'Neill, M.M. Mota, F. Lopes, R. Moreira (2013) "Structural optimization of quinolon-4(1H)-imines as dual-stage antimalarials: towards increased potency and metabolic stability", J. Med. Chem., 56, 7679-7690


53. T. Rodrigues, A.S. Ressurreição, F.P. da Cruz, I.S. Albuquerque, J. Gut, M. Carrasco, D. Gonçalves, M.M. Mota, P.J. Rosenthal, R. Moreira, M. Prudêncio, F. Lopes (2013) "Flavones as isosteres of 4(1H)-quinolones: discovery of ligand efficient and dual stage antimalarial lead compounds", Eur. J. Med. Chem., 69, 872-880


52. K. Hanson, A.S. Ressurreição, K. Buchholz, M. Prudêncio, J. Herman, M. Rebelo, W. Beatty, D. Wyrth, T. Hanscheid, R. Moreira, M. Marti, M.M. Mota (2013) "Torins are potent antimalarials that block replenishment of Plasmodium liver stage parasitophorous vacuole membrane proteins", Proc. Natl. Acad. Sci. USA, 110, E2838-E2847


51. T. Rodrigues, F.P. da Cruz, M. Lafuente-Monasterio, D. Gonçalves, A. Ressurreição, A.R. Sitoe, M.R. Bronze, J.Gut, G. Schneider, M.M. Mota, P.J. Rosenthal, M. Prudêncio, F.-J. Gamo, F. Lopes, R. Moreira (2013) "Quinolon-4(1H)-imines are Potent Antiplasmodial Drugs Targeting the Liver Stage of Malaria", J. Med. Chem., 56, 4811-4815

50. E. Dempsey, M. Prudêncio, B.J. Fennell, C.S. Gomes-Santos, J.W. Barlow, A. Bell (2013) "Antimitotic herbicides bind to an unidentified site on malarial parasite tubulin and block development of liver-stage Plasmodium parasites", Mol. Biochem. Parasitol., 188, 116-127. Highlighted as Feature Article on Malaria Nexus


49. J. Lin, P. Meireles, M. Prudêncio, S. Engelmann, T. Annoura, M. Sajid, S. Chevalley‐Maurel, J. Ramesar, C. Nahar, C.M.C. Avramut, A.J. Koster, K. Matuschewski, A.P. Waters, C.J. Janse, G.R. Mair, S.M. Khan (2013) "Loss‐of‐Function Analyses Defines Vital and Redundant Functions of the Plasmodium Rhomboid Protease Family", Mol. Microbiol., 88, 318-338


48. B.C. Pérez, C. Teixeira, I.S. Albuquerque, J. Gut, P.J. Rosenthal, J.R.B. Gomes, M. Prudêncio, P. Gomes (2013) "N-cinnamoylated chloroquine analogues as dual-stage antimalarial leads", J. Med. Chem., 56, 556-567


47. M. Prudêncio*, Maria M. Mota* (2013) "Targeting host factors to circumvent anti-malarial drug resistance", Curr. Pharm. Des., 19, 290-299 (Review) (* Corresponding authors)

46. B.C. Pérez, C. Teixeira, A.S. Gomes, I.S. Albuquerque, J. Gut, P.J. Rosenthal, M. Prudêncio, P. Gomes (2013) "In vitro efficiency of 9-(N-cinnamoylbutyl)aminoacridines against blood- and liver-stage malaria parasites", Bioorganic & Medicinal Chemistry Letters,  23, 610-613 

45. B.C. Pérez, C. Teixeira, I.S. Albuquerque, J. Gut, P.J. Rosenthal, M. Prudêncio* , P. Gomes* (2012) "PRIMACINS, N-cinnamoyl-primaquine conjugates with improved liver-stage antimalarial activity", Med. Chem. Commun., 3, 1170-1172 (* Corresponding authors) 


44. E.R. Derbyshire, M. Prudêncio, M.M. Mota, J. Clardy (2012) "Liver stage malaria parasites vulnerable to diverse chemical scaffolds", Proc. Natl. Acad Sci. USA, 109, 8511-8516


43. F.P. da Cruz, C. Martin, K. Buchholz K, M.J. Lafuente-Monasterio, T. Rodrigues, B. Sönnichsen, R. Moreira, F.J. Gamo, M. Marti, M.M. Mota, M. Hannus, M. Prudêncio* (2012) “Drug Screen Targeted at Plasmodium Liver Stages Identifies a Potent Multi-Stage Anti-Malarial Drug”, J. Inf. Dis., 205, 1278-1286, Evaluated by Faculty of 1000 Biology and Medicine  (* Corresponding author)

42. B.S.S. Guirgis, C.S. Cunha, I. Gomes, M. Cavadas, I. Silva, G. Doria, G.L. Blatch, P.V. Baptista, E. Pereira, H.M.E. Azzazy, M.M. Mota, M. Prudêncio, R. Franco (2012) ”Gold nanoparticle-based fluorescence immunoassay for malaria antigen detection”, Anal. Bioanal. Chem., 402, 1019-1027

41. J. Matos, F.P. da Cruz, E. Cabrita, J. Gut, F. Nogueira, V.E. do Rosário, R. Moreira, P.J. Rosenthal , M. Prudêncio, P. Gomes (2012) “Novel potent metallocenes against liver stage malaria”, Antimicrob. Agents Chemother., 56, 1564-1570

40. T. Rodrigues, M. Prudêncio, R. Moreira, M.M. Mota, F. Lopes (2012) “Targeting the Liver Stage of Malaria Parasites: A Yet Unmet Goal”, J. Med. Chem., 55, 995-1012

39. P. Eaton, V. Zuzarte-Luis, M.M. Mota, N.C. Santos, M. Prudêncio (2012) “Infection by Plasmodium changes shape and stiffness of hepatic cells”, Nanomedicine, 8, 17-19

38. C. Almeida, S. Kehraus, M. Prudêncio, G.M. König (2011) “Marilones A–C, phthalides from the sponge-derived fungus Stachylidium sp”, Beilstein J. Org. Chem., 7, 1636–1642

37. C. Ramalhete, F.P. da Cruz, D. Lopes, S. Mulhovo, V.E. Rosário, M. Prudêncio, M.J.U. Ferreira (2011) “Triterpenoids as inhibitors of erythrocytic and liver stages of Plasmodium infections”, Bioorg. Med. Chem., 19, 7474-7481

36. M. Prudêncio*, M.M. Mota, A.M. Mendes (2011) “A toolbox to study liver stage malaria”, Trends Parasitol., 27, 565-574 (Review), Selected by MalariaNexus  (* Corresponding author)


35. R. Capela, G.G. Cabal, P.J. Rosenthal, J. Gut, M.M. Mota, R. Moreira, F. Lopes, M. Prudêncio* (2011) “Design and evaluation of primaquine-artemisinin hybrids as a multistage antimalarial strategy”, Antimicrob. Agents Chemother., 55, 4698-4706  (* Corresponding author)

34. C.S.S. Gomes-Santos§, J. Braks§, M. Prudêncio , C. Carret, A.R. Gomes, A. Pain, T. Feltwell, S. Khan, A. Waters, C. Janse, G.R. Mair, M.M. Mota (2011) “Transition of Plasmodium Sporozoites into Liver Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio”, PloS Pathogens, 7(5):e1002046 (§ Equally contributing authors)

33. J. Lavrado, G.G. Cabal, M. Prudêncio, M.M. Mota, J. Gut, P.J. Rosenthal, C. Díaz, R.C. Guedes, D.J. Dos Santos, E. Bichenkova, K.T. Douglas, R. Moreira, A. Paulo (2011) “Incorporation of Basic Side Chains into Cryptolepine Scaffold: Structure-Antimalarial Activity Relationships and Mechanistic Studies”, J. Med. Chem., 54, 734-750

32. K. Slavic, M.J. Delves, M. Prudêncio, A.M. Talman, U. Straschil, E.T. Derbyshire, Z. Xu, R.E. Sinden, M.M. Mota, C. Morin, R. Tewari, S. Krishna, H.M. Staines (2011) “Use of a Selective Inhibitor To Define the Chemotherapeutic Potential of the Plasmodial Hexose Transporter in Different Stages of the Parasite's Life Cycle”, Antimicrob. Agents Chemother., 55, 2824-2830

31. P. Liehl, A.R. França, M. Prudêncio, E. Latz, A. Zaidman-Rémy, M.M. Mota (2010) “Phosphothioate Oligodeoxynucleotides inhibit Plasmodium sporozoite gliding motility”, Cell. Microbiol., 12, 506-515

30. I.H. Ploemen§, M. Prudêncio§, B.G. Douradinha, J. Ramesar, J. Fonager, G.J. van Gemert, A.J. Luty, C.C. Hermsen, R.W. Sauerwein, F.G. Baptista, M.M. Mota, A.P. Waters, I. Que, C.W. Lowik, S.M. Khan, C.J Janse, B.M. Franke-Fayard (2009) “Visualisation and quantitative analysis of the rodent malaria liver stage by real time imaging”, PLoS ONE, 4, e7881 (§ Equally contributing authors)

29. N. Vale, M. Prudêncio, C.A. Marques, M.S. Collins, J. Gut, F. Nogueira, J. Matos, P.J. Rosenthal, M.T. Cushion, V.E. do Rosário, M.M. Mota, R. Moreira, P. Gomes (2009) “Imidazoquines as Antimalarial and Antipneumocystis Agents”, J. Med. Chem., 52, 7800-7807

28. M. Prudêncio§, E.T. Derbyshire§, C.A. Marques, S. Krishna, M.M. Mota, H.M. Staines (2009) “Plasmodium berghei-infection induces volume-regulated anion channel-like activity in human hepatoma cells”, Cell. Microbiol., 11, 1492-1501 (§ Equally contributing authors)

27. S.S. Albuquerque, C. Carret, A.R. Grosso, A.S. Tarun, X. Peng, S.H.I. Kappe, M. Prudêncio, M.M. Mota (2009) “Host cell transcriptional profiling during malaria liver stage infection reveals a coordinated and sequential set of biological events”, BMC Genomics, 10, 270-282

26. M. Prudêncio, M.J. Lehmann (2009) “Illuminating the host - How RNAi screens shed light on host-pathogen interactions”, Biotechnol. J., 4, 826-837

25. I. Parvanova, S. Epiphanio, A. Fauq, T.E. Golde, M. Prudêncio, M.M. Mota (2009) “A Small Molecule Inhibitor of Signal Peptide Peptidase Inhibits Plasmodium Development in the Liver and Decreases Malaria Severity”, PLoS ONE, 4, e5078

24. M. Prudêncio§, C.D. Rodrigues§, M. Hannus, C. Martin, E. Real, L .A. Gonçalves, C. Carret, R. Dorkin, I. Röhl, K. Jahn-Hoffmann, A.J.F. Luty, R. Sauerwein, C.J. Echeverri, M.M. Mota (2008) “Kinome-Wide RNAi Screen Implicates at Least 5 Host Hepatocyte Kinases in Plasmodium Sporozoite Infection”, PloS Pathogens, 4, 1-15 (§ Equally contributing authors)


23. M.G. Finiguerra, , M. Ubbink, M. Huber (2008) “Accurate Long-Range Distance Measurements in a Doubly Spin-Labeled Protein by a Four-Pulse, Double Electron-Electron Resonance Method”, Magn. Reson. Chem., 46, 1096-1101

22. C.D. Rodrigues§, M. Hannus§, M. Prudêncio§, C. Martin, L.A. Gonçalves, S. Portugal, S. Epiphanio, A. Akinc, P. Hadwiger, K. Jahn-Hofmann, I. Röhl, G.J. van Gemert, J.F. Franetich, A.J. Luty, R. Sauerwein, D. Mazier, V. Koteliansky, H.P. Vornlocher, C.J. Echeverri, M.M. Mota (2008) “Host Scavenger Receptor SR-BI Plays a Dual Role in the Establishment of Malaria Parasite Liver Infection”, Cell Host & Microbe, 11, 4, 271-282, ) § Equally contributing authors)

21. O. Silvie, M.M. Mota, K. Matuschewski,  M. Prudêncio (2008) “Interactions of the malaria parasite and its mammalian host”, Curr. Opin. Microbiol., 11, 352-359 (Review)

20. M. Cunha-Rodrigues§, S. Portugal§, M. Prudêncio, L.A. Gonçalves, C. Casalou, D. Buger, R. Sauerwein, W. Haas, M.M. Mota (2008) “Genistein-supplemented diet decreases malaria liver infection in mice and constitutes a potential prophylactic strategy”, PLoS ONE, 3, e2732 (§ Equally contributing authors)

19. M.D. Vlasie, R. Fernández-Busnadiego, M. Prudêncio, M. Ubbink (2008) “Conformation of Pseudoazurin in the 152 kDa Electron Transfer Complex with Nitrite Reductase Determined by Paramagnetic NMR”*; J. Mol. Biol., 375, 1405-1415 (*Issue cover)

18. M. Prudêncio*, C.D. Rodrigues, R. Ataíde, M.M. Mota* (2008), “Dissecting in vitro host cell infection by Plasmodium sporozoites using flow cytometry”, Cell. Microbiol., 10, 218-224 (* Corresponding authors)

17. M. Prudêncio, M.M. Mota (2007), “To migrate or to invade: those are the options”, Cell Host & Microbe, 2, 286-288 (Review)

16. M. D. Vlasie, C. Comuzzi, A. M. C. H. van den Nieuwendijk, M. Prudêncio, M. Overhand, M. Ubbink (2007), “Long-range-distance NMR effects in a protein labeled with a lanthanide–DOTA chelate”, Chem. Eur. J., 13, 1715 – 1723

15. T.E. de Jongh, A.-M. van Roon, M. Prudêncio, M. Ubbink, G.W. Canters (2006), “Click chemistry with an active site variant of azurin”, Eur. J. Inorg. Chem., 3861-3868

14. M. Prudêncio, A. Rodriguez, M.M. Mota (2006), “The silent path to thousands of merozoites: the Plasmodium liver stage”, Nat. Rev. Microbiol., 4, 849-856 (Review)

13. M. Cunha-Rodrigues, M. Prudêncio, M.M. Mota, W. Haas (2006) “Antimalarial drugs - host targets (re)visited”, Biotechnol. J., 1, 321-332 (Review)

12. J.A. Worrall, R.E. Diederix, M. Prudêncio, C.E. Lowe, S. Ciofi-Baffoni, M. Ubbink, G.W. Canters (2005) “The effects of ligand exchange and mobility on the peroxidase activity of a bacterial cytochrome c upon unfolding”, ChemBioChem, 6, 747-758

11. R.L. Harris, M. Prudêncio, S.S. Hasnain, R.R. Eady and G. Sawers (2005) “Heterologous metalloprotein biosynthesis in Escherichia coli: conditions for the overproduction of functional copper-containing nitrite reductase and azurin from Alcaligenes xylosoxidans”, J. Synchrotron Rad., 12, 13–18

10. M. Prudêncio, M. Ubbink (2004) “Transient Complexes of Redox Proteins: Structural and Dynamic Details from NMR Studies”, J. Mol. Recognit., 17, 524-539 (Review)

9. M. Prudêncio, J. Rohovec, J.A. Peters, E. Tocheva, M.J. Boulanger, M.E.P. Murphy, H.-J. Hupkes, W. Kosters, A. Impagliazzo, M. Ubbink (2004) “A Caged Lanthanide Complex as a Paramagnetic Shift Agent for Protein NMR”, Chem. Eur. J., 10, 3252-3260

8. O. Farver, R.R. Eady, G. Sawers, M. Prudêncio, I. Pecht (2004) “Met144Ala Mutation of the Copper-Containing Nitrite Reductase from Alcaligenes xylosoxidans reverses the Intramolecular Electron Transfer”, FEBS Lett., 561, 173-176


7. M. Prudêncio, G. Sawers, S.A. Fairhurst, F.K. Yousafzai, R.R. Eady (2002) “Alcaligenes xylosoxidans Dissimilatory Nitrite Reductase: Alanine Substitution of the Surface-exposed Histidine 139 Ligand of the Type 1 Copper Center Prevents Electron Transfer to the Catalytic Center”, Biochemistry, 41, 3430-3438

6. M.J. Ellis, M. Prudêncio, F.E. Dodd, R.W. Strange, G. Sawers, R.R. Eady, S.S. Hasnain (2002) “Biochemical and Crystallographic Studies of the Met144Ala, Asp92Asn and His254Phe Mutants of the Nitrite Reductase from Alcaligenes xylosoxidans Provide Insight into the Enzyme Mechanism”, J. Mol. Biol., 316, 51-64

5. M.J. Ellis, F.E. Dodd, R.W. Strange, M. Prudêncio, G. Sawers, R.R. Eady, S.S. Hasnain (2001) “X-ray Structure of a Blue Copper Nitrite Reductase at High pH and in Copper-free Form at 1.9 Angstrom Resolution”, Acta Crystallogr. D, 57, 1110-1118

4. M. Prudêncio, R.R. Eady, G. Sawers (2001) “Catalytic and Spectroscopic Analysis of Blue Copper-containing Nitrite Reductase Mutants Altered in the Environment of the Type 2 Copper Centre: Implications for Substrate Interaction”, Biochem. J., 353, 259-266

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